Widal antibody titre test versus blood culture; which is a better diagnostic for typhoid fever?

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چکیده

Background: The importance of accurate diagnosis infectious diseases is central and crucial to the effectiveness treatment prevention associated long-term complications such infections. objective this study was therefore determine accuracy Widal antibody titre test in typhoid fever relative gold standard blood culture technique.Methodology: A total 40 students attending Olabisi Onabanjo University Health Services, Ago-Iwoye, Ogun State, Nigeria on account suspected by positive (≥ 1/80) not antibiotic therapy, were recruited for study. Stool samples collected from each participant analysed at medical laboratory health center using conventional techniques confirmation isolates simplex multiplex polymerase chain reaction (PCR) amplification assays hilA (Salmonella enterica), ipaH (Shigella spp), rfc flexneri) wbgZ sonnei) genes. Antibiotic susceptibility testing (AST) isolated bacteria 10 panel antibiotics done Kirby Bauer disk diffusion interpreted according Clinical Laboratory Standards Institute (CSLI) guideline.Results: Of patients with test, 9 yielded Salmonella enterica giving a 22.5% isolation rate, serovar Typhi Typhi) confirmed as sole bacterium cultures 5 (12.5%) co-infection Shigella stool 4 (10.0%) patients. 52 enteric recovered made (17.3%), spp 20 (38.5%), S. flexneri (17.3%) sonnei 14 (26.9%). All multi-drug resistant (MDR), resistance rates ranging 33.3%-100%, all ceftriaxone pefloxacin. also 100% nitrofurantoin, ofloxacin ciprofloxacin; amoxicillin, cotrimoxazole, nitrofurantoin cotrimoxazole.Conclusion: These results showed that only 12.5% had their while other cases. There need adopt febrile illnesses order improve regimen. fact AST can be performed technique could further guide prescription reduce risk emergence bacteria.
 Contexte: L'importance d'un diagnostic précis des maladies infectieuses est centrale et cruciale pour l'efficacité du traitement la prévention à long terme associées ces L'objectif de cette étude était donc déterminer précision d'anticorps dans le fièvre typhoïde par rapport d'hémoculture référence.Méthodologie : Un étudiants fréquentant les services santé l'Université Onabanjo, État d'Ogun, Nigéria en raison d'une suspectée un positif non sous antibiothérapie, ont été recrutés l'étude. Des échantillons selles sang prélevés sur chaque analysés au laboratoire médical centre l'aide conventionnelles isolats tests d'amplification réaction chaîne polymérase gènes sonnei). Les sensibilité aux antibiotiques bactéries isolées groupes d'antibiotiques effectués disque interprétés conformément directives (CSLI).Résultats: Sur suspects Widal, révélé donnant taux d'isolement 22,5%, avec sérovar confirmée comme bactérie unique partir d'hémocultures chez (12,5%) d'échantillons (10,0%) entériques étérécupérés constitués (17,3%), (38,5%), (17,3%) (26,9%). Tous étaient multirésistants résistance allant 33,3% 100%, tous résistants péfloxacine. également nitrofurantoïne, l'ofloxacine ciprofloxacine; résistant l'amoxicilline, nitrofurantoïne Ces résultats montré que seuls 12,5% diagnostics avaient isolée leurs hémocultures, tandis d'autres cas. Il nécessaire d'adopter fébriles afin d'améliorer schéma thérapeutique. Le fait l'AST puisse être réalisée une pourrait guider davantage réduire risque d'émergence résistantes.

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ژورنال

عنوان ژورنال: African Journal of Clinical and Experimental Microbiology

سال: 2022

ISSN: ['1595-689X']

DOI: https://doi.org/10.4314/ajcem.v23i4.7